2025 Midwest Fish & Wildlife Conference

AUTHORS: Brittany L. Harried, Missouri Cooperative Fish and Wildlife Research Unit, School of Natural Resources, University of Missouri, Columbia, MO; Jacob T. Westhoff, U.S. Geological Survey, Missouri Cooperative Fish and Wildlife Research Unit, School of Natural Resources, University of Missouri, Columbia, MO

ABSTRACT: The Bluestripe Darter (Percina cymatotaenia) is endemic to Missouri, is a species of conservation concern, and has been petitioned for listing under the Endangered Species Act. We conducted a study to evaluate the species’ threats, genetic diversity, and population trends. Objectives for this study were to: 1) duplicate Bluestripe Darter sampling effort from past sites and observe trends in population status, and 2) assess the range-wide geographic patterns of genetic variation within Bluestripe Darter. Sampling efforts from 2000-2002 were duplicated during June-August 2024 for 46 sites across five rivers in southern Missouri using seining methods that targeted small-bodied benthic fishes. Furthermore, limited environmental covariate and habitat data were collected and additional sampling in a spatially replicated occupancy framework was completed to estimate occupancy and detection at each site. Up to 20 fin clips and vouchers were collected per river to estimate effective population sizes, geographic structure of genetic variation within and between drainages, and contemporary short-term migration rates. Photos of each voucher specimen were also taken to assess external ecomorphology. We detected Bluestripe Darter at 9 out of 46 sites in 2024, which was well below the 23 out of 46 sites where it was detected by surveys from 2000-2002. Results from this study will inform the Species Status Assessment and listing decision for Bluestripe Darter, and will also benefit Heritage reviews, Wildlife Collector Permit reviews, conservation planning, and priority for on-the ground management projects related to identified population threats.